MersV1, Main, Exploration, bibRecord, 003976

A Reverse Transcriptase/Maturase Promotes Splicing by Binding at Its Own Coding Segment in a Group II Intron RNA

Identifieur interne : 003976 ( Main/Exploration ); précédent : 003975; suivant : 003977

A Reverse Transcriptase/Maturase Promotes Splicing by Binding at Its Own Coding Segment in a Group II Intron RNA

Auteurs : Herbert Wank [États-Unis] ; Joseph Sanfilippo [États-Unis] ; Ravindra N. Singh [États-Unis] ; Manabu Matsuura [États-Unis] ; Alan M. Lambowitz [États-Unis]

Source :

Molecular Cell [ 1097-2765 ] ; 1999.

RBID : ISTEX:6A63F089BEA60637B3A210F008B0588DC4D785B1

English descriptors

Teeft :
- Assay, Binding site, Cdna, Cdna synthesis, Codon, Deletion, Different combinations, Domain, Endonuclease, Exon, Exon sequences, Intron, Intron maturase, Intron mobility, Koff, Koff measurements, Lambowitz, Lariat, Ltra, Ltra protein, Matsuura, Maturase, Mgcl2, Mobile group, Molar ratio, Molecular cell, Mutation, Nacl, Other regions, Precursor, Primary binding site, Primer, Reaction medium, Residual, Rna, Saldanha, Secondary contacts, Secondary structure, Similar results, Specific binding, Tight binding, Tprt, Transcriptase, Transcription, Unspliced, Unspliced precursor, Zimmerly.

Abstract

Abstract: Group II introns encode reverse transcriptases that promote RNA splicing (maturase activity) and then with the excised intron form a DNA endonuclease that mediates intron mobility by target DNA-primed reverse transcription (TPRT). Here, we show that the primary binding site for the maturase (LtrA) encoded by the Lactococcus lactis Ll.LtrB intron is within a region of intron domain IV that includes the start codon of the LtrA ORF. This binding is enhanced by other elements, particularly domain I and the EBS/IBS interactions, and helps position LtrA to initiate cDNA synthesis in the 3′ exon as occurs during TPRT. Our results suggest how the maturase functions in RNA splicing and support the hypothesis that the reverse transcriptase coding region was derived from an independent genetic element that was inserted into a preexisting group II intron.

Url:

https://api.istex.fr/ark:/67375/6H6-PTT7S3L5-3/fulltext.pdf

DOI: 10.1016/S1097-2765(00)80371-8

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 002707
to stream Istex, to step Curation: 002707
to stream Istex, to step Checkpoint: 001230
to stream Main, to step Merge: 003A21
to stream Main, to step Curation: 003976

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Reverse Transcriptase/Maturase Promotes Splicing by Binding at Its Own Coding Segment in a Group II Intron RNA</title>
<author><name sortKey="Wank, Herbert" sort="Wank, Herbert" uniqKey="Wank H" first="Herbert" last="Wank">Herbert Wank</name>
</author>
<author><name sortKey="Sanfilippo, Joseph" sort="Sanfilippo, Joseph" uniqKey="Sanfilippo J" first="Joseph" last="Sanfilippo">Joseph Sanfilippo</name>
</author>
<author><name sortKey="Singh, Ravindra N" sort="Singh, Ravindra N" uniqKey="Singh R" first="Ravindra N." last="Singh">Ravindra N. Singh</name>
</author>
<author><name sortKey="Matsuura, Manabu" sort="Matsuura, Manabu" uniqKey="Matsuura M" first="Manabu" last="Matsuura">Manabu Matsuura</name>
</author>
<author><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:6A63F089BEA60637B3A210F008B0588DC4D785B1</idno>
<date when="1999" year="1999">1999</date>
<idno type="doi">10.1016/S1097-2765(00)80371-8</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-PTT7S3L5-3/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002707</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002707</idno>
<idno type="wicri:Area/Istex/Curation">002707</idno>
<idno type="wicri:Area/Istex/Checkpoint">001230</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001230</idno>
<idno type="wicri:doubleKey">1097-2765:1999:Wank H:a:reverse:transcriptase</idno>
<idno type="wicri:Area/Main/Merge">003A21</idno>
<idno type="wicri:Area/Main/Curation">003976</idno>
<idno type="wicri:Area/Main/Exploration">003976</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A Reverse Transcriptase/Maturase Promotes Splicing by Binding at Its Own Coding Segment in a Group II Intron RNA</title>
<author><name sortKey="Wank, Herbert" sort="Wank, Herbert" uniqKey="Wank H" first="Herbert" last="Wank">Herbert Wank</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, Texas 78712</wicri:regionArea>
<orgName type="university">Université du Texas à Austin</orgName>
<placeName><settlement type="city">Austin (Texas)</settlement>
<region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sanfilippo, Joseph" sort="Sanfilippo, Joseph" uniqKey="Sanfilippo J" first="Joseph" last="Sanfilippo">Joseph Sanfilippo</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, Texas 78712</wicri:regionArea>
<orgName type="university">Université du Texas à Austin</orgName>
<placeName><settlement type="city">Austin (Texas)</settlement>
<region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Singh, Ravindra N" sort="Singh, Ravindra N" uniqKey="Singh R" first="Ravindra N." last="Singh">Ravindra N. Singh</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, Texas 78712</wicri:regionArea>
<orgName type="university">Université du Texas à Austin</orgName>
<placeName><settlement type="city">Austin (Texas)</settlement>
<region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsuura, Manabu" sort="Matsuura, Manabu" uniqKey="Matsuura M" first="Manabu" last="Matsuura">Manabu Matsuura</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, Texas 78712</wicri:regionArea>
<orgName type="university">Université du Texas à Austin</orgName>
<placeName><settlement type="city">Austin (Texas)</settlement>
<region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name>
<affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, Texas 78712</wicri:regionArea>
<orgName type="university">Université du Texas à Austin</orgName>
<placeName><settlement type="city">Austin (Texas)</settlement>
<region type="state">Texas</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Molecular Cell</title>
<title level="j" type="abbrev">MOLCEL</title>
<idno type="ISSN">1097-2765</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1999">1999</date>
<biblScope unit="volume">4</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="239">239</biblScope>
<biblScope unit="page" to="250">250</biblScope>
</imprint>
<idno type="ISSN">1097-2765</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1097-2765</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term>
<term>Binding site</term>
<term>Cdna</term>
<term>Cdna synthesis</term>
<term>Codon</term>
<term>Deletion</term>
<term>Different combinations</term>
<term>Domain</term>
<term>Endonuclease</term>
<term>Exon</term>
<term>Exon sequences</term>
<term>Intron</term>
<term>Intron maturase</term>
<term>Intron mobility</term>
<term>Koff</term>
<term>Koff measurements</term>
<term>Lambowitz</term>
<term>Lariat</term>
<term>Ltra</term>
<term>Ltra protein</term>
<term>Matsuura</term>
<term>Maturase</term>
<term>Mgcl2</term>
<term>Mobile group</term>
<term>Molar ratio</term>
<term>Molecular cell</term>
<term>Mutation</term>
<term>Nacl</term>
<term>Other regions</term>
<term>Precursor</term>
<term>Primary binding site</term>
<term>Primer</term>
<term>Reaction medium</term>
<term>Residual</term>
<term>Rna</term>
<term>Saldanha</term>
<term>Secondary contacts</term>
<term>Secondary structure</term>
<term>Similar results</term>
<term>Specific binding</term>
<term>Tight binding</term>
<term>Tprt</term>
<term>Transcriptase</term>
<term>Transcription</term>
<term>Unspliced</term>
<term>Unspliced precursor</term>
<term>Zimmerly</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Group II introns encode reverse transcriptases that promote RNA splicing (maturase activity) and then with the excised intron form a DNA endonuclease that mediates intron mobility by target DNA-primed reverse transcription (TPRT). Here, we show that the primary binding site for the maturase (LtrA) encoded by the Lactococcus lactis Ll.LtrB intron is within a region of intron domain IV that includes the start codon of the LtrA ORF. This binding is enhanced by other elements, particularly domain I and the EBS/IBS interactions, and helps position LtrA to initiate cDNA synthesis in the 3′ exon as occurs during TPRT. Our results suggest how the maturase functions in RNA splicing and support the hypothesis that the reverse transcriptase coding region was derived from an independent genetic element that was inserted into a preexisting group II intron.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Texas</li>
</region>
<settlement><li>Austin (Texas)</li>
</settlement>
<orgName><li>Université du Texas à Austin</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Texas"><name sortKey="Wank, Herbert" sort="Wank, Herbert" uniqKey="Wank H" first="Herbert" last="Wank">Herbert Wank</name>
</region>
<name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name>
<name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name>
<name sortKey="Matsuura, Manabu" sort="Matsuura, Manabu" uniqKey="Matsuura M" first="Manabu" last="Matsuura">Manabu Matsuura</name>
<name sortKey="Sanfilippo, Joseph" sort="Sanfilippo, Joseph" uniqKey="Sanfilippo J" first="Joseph" last="Sanfilippo">Joseph Sanfilippo</name>
<name sortKey="Singh, Ravindra N" sort="Singh, Ravindra N" uniqKey="Singh R" first="Ravindra N." last="Singh">Ravindra N. Singh</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003976 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003976 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:6A63F089BEA60637B3A210F008B0588DC4D785B1
   |texte=   A Reverse Transcriptase/Maturase Promotes Splicing by Binding at Its Own Coding Segment in a Group II Intron RNA
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

A Reverse Transcriptase/Maturase Promotes Splicing by Binding at Its Own Coding Segment in a Group II Intron RNA

A Reverse Transcriptase/Maturase Promotes Splicing by Binding at Its Own Coding Segment in a Group II Intron RNA

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri